Skip Navigation

Neuro-Oncology 2009 11(2):122-131; doi:10.1215/15228517-2008-085
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Siegelin, M. D.
Right arrow Articles by von Deimling, A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Siegelin, M. D.
Right arrow Articles by von Deimling, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Copyright 2009 by the Society for Neuro-Oncology

Quercetin promotes degradation of survivin and thereby enhances death-receptor–mediated apoptosis in glioma cells

Markus D. Siegelin, David E. Reuss, Antje Habel, Abdelhaq Rami and Andreas von Deimling

Department of Neuropathology, University Hospital Heidelberg, Heidelberg (M.D.S., D.E.R., A.H., A.V.); Institute for Cellular and Molecular Anatomy III, Department of Medicine, Johann Wolfgang von Goethe University Hospital, Frankfurt (A.R.); Clinical Cooperation Unit Neuropathology, German Cancer Center (DKFZ), Heidelberg (A.V.); Germany

Address correspondence to Andreas von Deimling, Department of Neuropathology, University Hospital Heidelberg, Im Neuenheimer Feld 220, 69120 Heidelberg, Germany (andreas.vondeimling{at}med.uni-heidelberg.de).


  Abstract

The flavonoid quercetin has been reported to inhibit the proliferation of cancer cells, whereas it has no effect on nonneoplastic cells. U87-MG, U251, A172, LN229, and U373 malignant glioma cells were treated with quercetin (50–200 µM). Quercetin did not cause cytotoxicity 24 h after treatment. Combining quercetin with tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) strongly augmented TRAIL-mediated apoptosis in U87-MG, U251, A172, and LN229 glioma cells; U373 cells could not be sensitized by quercetin to TRAIL-mediated apoptosis. TRAIL-induced apoptosis was enhanced by quercetin-induced reduction of survivin protein levels. Upon treatment with quercetin, the protein level of survivin was strongly suppressed in U87-MG, U251, and A172 but not in U373 glioma cells. Quercetin exposure resulted in proteasomal degradation of survivin. TRAIL-quercetin–induced apoptosis was markedly reduced by overexpression of survivin. In addition, upon treatment with quercetin, downregulation of survivin was also regulated by the Akt pathway. Taken together, the results of the present study suggest that quercetin sensitizes glioma cells to death-receptor–mediated apoptosis by suppression of inhibitor of the apoptosis protein survivin.

Keywords: apoptosis, glioma, quercetin, survivin, TRAIL

Received November 12, 2007; Accepted April 29, 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
B. Romani, S. Engelbrecht, and R. H. Glashoff
Functions of Tat: the versatile protein of human immunodeficiency virus type 1
J. Gen. Virol., January 1, 2010; 91(1): 1 - 12.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.