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Neuro-Oncology 2002 4(3):165-170; doi:10.1093/neuonc/4.3.165
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© 2002 by the Society forNeuro-Oncology

Medulloblastomas and primitive neuroectodermal tumors rarely containpolyomavirus DNA sequences

John Y.H. Kim, Igor J. Koralnik, Mark LeFave, Rosalind A. Segal, Luz-Andrea Pfister and Scott L. Pomeroy2

Division of Neuroscience (J.Y.H.K., M.L.,S.L.P.), Department of Neurology (S.L.P.), Children'sHospital; Department of Neurology (I.J.K.) and Division of Viral Pathogenesis (I.J.K., L.-A.P.), BethIsrael Deaconess Medical Center; and Department ofPediatric Oncology, Dana-Farber Cancer Institute (J.Y.H.K., R.A.S.), HarvardMedical School, Boston, MA 02115

2 Address correspondence and reprint requests to Scott L. Pomeroy, Department ofNeurology, Children's Hospital, 300 Longwood Ave., Boston, MA 02115.


  Abstract

To address the hypothesis that medulloblastoma or supratentorial primitiveneuroectodermal tumor (sPNET) can arise through infection by polyomaviruses,we examined genomic DNA isolated from 15 primary medulloblastoma and 5 sPNETbiopsy specimens and from 2 medulloblastoma cell lines for the presence of DNAsequences from the polyomaviruses simian virus 40 (SV40), JC virus, and BKvirus. These polyomaviruses have oncogenic potential in animals, and their DNAsequences have been detected in other surveys of various solid tumors,including childhood brain tumors. The tumor DNA samples were analyzed bySouthern blot hybridization of polymerase chain reaction products thatemployed probes designed to detect specific polyomavirus sequences. Neither JCvirus nor BK virus DNA sequences were detected in any of the specimens. Noneof the primary medulloblastoma or sPNET specimens contained SV40 sequences.However, SV40 DNA coding and noncoding sequences were detected in the D283-Med(medulloblastoma) cell line. Immunocytochemical studies of D283-Med revealednuclear expression of SV40 large T antigen. In contrast to childhoodependymomas and choroid plexus tumors, medulloblastomas and sPNETsinfrequently express evidence of polyomavirus infection.

Received November 7, 2001; Accepted February 6, 2002


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