Skip Navigation

Neuro-Oncology 2004 6(2):83-95; doi:10.1215/S1152851703000140
© 2004 by Society
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gomez, G. G.
Right arrow Articles by Kruse, C. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Gomez, G. G.
Right arrow Articles by Kruse, C. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004 by the Society forNeuro-Oncology

Interactions of the allogeneic effector leukemic T cell line, TALL-104,with human malignant brain tumors

German G. Gomez, Susana B. Read, Lazaro E. Gerschenson, Daniela Santoli, Adam Zweifach and Carol A. Kruse2

Departments of Pathology (G.G.G., L.E.G.,C.A.K.), Immunology (S.B.R., A.Z., C.A.K.), and Physiology (A.Z.), University of Colorado Health SciencesCenter, Denver, CO 80262, and The Wistar Institute(D.S.), Philadelphia, PA 19104; USA

2 Address correspondence to Carol A. Kruse, Department of Immunology, Campus BoxB216, University of Colorado Health Sciences Center, 4200 East Ninth Avenue,Denver, CO 80262(carol.kruse{at}uchsc.edu).


  Abstract

TALL-104 is a human leukemic T cell line that expresses markerscharacteristic of both cytotoxic T lymphocytes and natural killer cells.TALL-104 cells are potent tumor killers, and the use of lethally irradiatedTALL-104 as cellular therapy for a variety of tumors has been explored. Weinvestigated the interactions of TALL-104 cells with human brain tumor cells.TALL-104 cells mediated increased lysis of a panel of brain tumor cells at loweffector-to-target ratios over time. We obtained evidence that TALL-104 cellsinjured glioma cells by both apoptotic and necrotic pathways. A 7-aminoactinomycin D flow cytometry assay revealed that the percentages of bothapoptotic and necrotic glioma cells increased after TALL-104 cell/glioma cellcoincubations. Fluorescent microscopy studies and a quantitative morphologicassay confirmed that TALL-104 cell/glioma cell interactions resulted in tumorcell apoptosis. Cytokines are secreted when TALL-104 cells are coincubatedwith brain tumor cells; however, morphologic analysis assays revealed that thesoluble factors contained within clarified supernates obtained from 4 hcoincubates added back to brain tumor cell cultures did not trigger the gliomaapoptosis. TALL-104 cells do not express Fas ligand, even upon coincubationwith glioma targets, which suggests that the Fas/Fas ligand apoptotic pathwayis not likely responsible for the cell injury observed. We obtained evidencethat cell injury is calcium dependent and that lytic granule exocytosis istriggered by contact of TALL-104 cells with human glioma cells, suggestingthat this pathway mediates glioma cell apoptosis and necrosis.

Received April 23, 2003; Accepted October 10, 2003


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.